ID - AFB Slide TECHNICAL INFORMATION INTENDED USE ID-AFB slide is a multi-purpose microscope slide recommended to be part of a quality control program to monitor stains and techniques. PRINCIPLES The slide contains one oval (+) with an acid fast positive control (Mycobacterium gordonae ATCC 14470). The slide also contains one oval (-)            with an acid fast negative control (Eschenchia coli ATCC 25922). The slide contains a blank oval for the convenience of the technologist to                  be utilized in performing clinical AFB stains. MATERIALS PRECAUTIONS: This product is for IN VITRO DIAGNOSTIC USE only. This product may contain potentially viable organisms. Handle it as you would a known pathogen. STORAGE: Do not freeze or expose to excessive heat. Store at room temperature and do not use beyond expiration date. PROCEDURE: MATERIALS REQUIRED BUT NOT PROVIDED: The standard clinical microbiological equipment such as loop, burner, stains are       needed for procedures involving the use of this product. 1. Place the specimen to be examined within confines of the blank oval. 2. Air dry and heat fix by gentle heating over a bunsen bumer flame. 3. Perform AFB stain: a. Flood entire stain with Kinyoun Carbol Fuchsin for 3 minutes. b. Wash gently in running water, rinsing both sides. c. Decolorize with acid alcohol until all of the color is washed out (about 1-2 minutes depending on the thickness   of the smear). d. Wash gentiy in running water rinsing both sides. e. Counterstain with methylene blue or malachite green for 30 seconds. f. Wash gently in running water rinsing both sides. g. Allow slide to air dry. Do not blot. h. Examine under microscope using oil immersion lens. EXPECTED RESULTS Positive control: red stained rod-shaped or coccobacillary bactena (from 0.5 to 5.0 x 0.2 to 0.6 microns). Negative control: No red-stained bacteria. Acid fast positive organisms stain red. Acid fast negative organisms stain blue or green (depending on counterstain) Note: It is possible that positive control organisms can carry over to the patient testing area. As a precaution, if a positive patient result is recorded, repeat the testing process on a clean slide that does not contain a positive control organism. LIMITATIONS OF PROCEDURE Acid-fast bacteria may become nonacid-fast if they are exposed to ultraviolet light, direct sunlight, or overheating. Saprophytic acid4ast bacteria          are found in soil and water and may contaminate specimens during processing, resulting in a false-positive test. (3) Heat-fixing does not always kill mycobacteria and the specimen smear may be a potential source of infection. REFERENCES 1. Smithwick, R.W. 1976. Laboratory manual for acid4ast microscopy, 2nd ed. Centers for Disease Control, Atlanta. 2. Vestal, A.L. 1978. Procedures for the isolation and identification of mycobacteria. HEW Publication No. (CDC) 79-8230. Centers                   for Disease Control, Atlanta. 3. Dizon, D., C. Mihailescu, and H.C. Cuthbert. 1976. Simple procedure for detection of Mycobacterium gordonae in water causing              false-positive add4ast smears. J. Clin. Microbiol. 3:211.

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