COLIFORM ISOLATION TRANSWAB®

For the early detection of E. coli in the environment

 

 Escherichia coli and Coliforms are traditionally used as indicator organisms for fecal contamination in water and other environmental samples. Detection of these organisms usually points to poor hygiene at some stage in the production process or pollution of water at the source.

 Based on a traditional test for Coliforms, the Coliform  Isolation Transwab® provides a simple method for detecting these organisms—showing a definite color change from purple to yellow when Coliforms are present.

 

INSTRUCTIONS FOR USE  

1.        Peel back wrap to expose both caps

2.        Remove swab and sample test site

3.        Remove cap of culture tube with thumb and forefinger and discard

4.        Insert swab in culture tube  and push down fully to immerse swab completely

5.        Fill time, date, and site details

6.        Incubate 37° C  for 18-24 hours

7.        Read results. A positive  result is indicated by a color change from purple to yellow.

       A negative result will show no color change

8.        Record results and dispose of tube

 

PRECAUTIONS WHEN SWABBING

1.        When sampling an area always use the same swabbing technique

2.        Always try to sample the same surface area (100cm² or  200cm²), be consistent in your sampling

3.        The moisture level on the surface should not vary at different sample times. It may be preferable with dry surfaces to wet the swab in a suitable wetting agent such as Ringes, Tween Lecithin or Tween Peptone Water

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COMPETITIVE COMPARISON SHEET

 RODAC PLATES/CONTACT SLIDES (VRBA or VRBGA)                    COLIFORM TRANSWAB®

1          Six months shelf-life or less                                                       1 year shelf-life

2          Refrigerated Storage                                                                 Room Temperature Storage

3          Reading results time consuming & misinterpretations               Simple Color Change Indicating Result

4          Wet surfaces can cause swarming on plates & unreadable       Wet surfaces do not effect

                                                                                                               and & can even enhance

5          Rough uneven surface can not sampled properly                      Rough or uneven surfaces do not

                                                                                                                affect sampling

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 APPLICATIONS

 FISHING INDUSTRY,  MEAT INDUSTRY,  DAIRY INDUSTRY, POULTRY INDUSTRY, PROCESSED FOODS

SHELF-LIFE

  The expiry date is 12 months from the month of manufacture and is printed on the tube label, Peel Pouch and outer box

 STORAGE

  Coliform Isolation Transwab® should be stored in a dry area between +5° C to +25° C.  DO NOT FREEZE

 STERILIZATION  method is by irradiation

 REFERENCES

 1.         Ref Number: 000262

         Murphy F. Tanker  Hygiene Control 1993.

          Key Words:  MW%)#; MW504; MWE: E. coli; Coliform

2.         Ref Number 00392

        Lockett, c. An In-House comparative study of MW503 Coliform Isolation Transwab and 

          MW504 Hygiene Test at  two  incubation temperatures. 1984

 

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