INDIA INK CONTROL
India Ink Control is recommended to be part of
quality control to monitor India Ink wet mount
SUMMARY AND EXPLANATION
India Ink wet mounts are used for the
visualization of encapsulated microorganisms,
especially Cryptococcus neoformans,
directly in clinical specimens. The
polysaccharide capsules of organisms will
exclude the particles of ink, and the capsules
will appear as clear haloes around the organisms
on semi-opaque backgrounds.
The India Ink Positive Control consists of a
suspension of encapsulated Cryptococcus
neoformans as a positive control for India
Ink wet mount preparations. A suspension of
non-encapsulated yeasts is provided as a
negative control for India Ink wet mount
Positive: Cryptococcus neoformans
Negative: Yeasts (non-encapsulated suspension)
This medium is for IN VITRO DIAGNOSTIC USE only.
Precautions should be taken against the dangers
of microbiological hazards. Specimens,
containers, and media should be sterilized after
use. This product may contain potentially viable
organisms. Handle it as you would a known
Media should be stored at room temperature in
the dark and remain in the original packaging
EVIDENCE OF DETERIORATION
This medium should not be used if the expiration
date has passed. If any deficiencies are
observed, notify the manufacturer.
OTHER MATERIAL REQUIRED BUT NOT SUPPLIED
The usual clinical microbiological equipment
such as India Ink, Nigrosin, slides, cover slips,
and microscope, are needed for procedures
involving the use of this product.
1. Place equal parts of the India Ink Control
suspension and India Ink (or Nigrosin) on the
2. Mix well, and add a cover slip.
3. Examine at x100 to x1,000.
The India Ink Positive Control contains capsules
that will exclude the ink and appear as clear
haloes around the organism.
LIMITATION OF THE PROCEDURES
Results may be hindered if the emulsion with the
suspension is too thick, blocking the
transmission of light completely.
1. Murray, P.R. et al. 1995. Manual of Clinical
Microbiology, 6th ed. ASM. Washington, DC.