ID - UREASE  - CAT # 60045 PRODUCT TECHNICAL INFORMATION USE The Gibson ID - Urease is designed for the detection of urease activity in a variety of microorganisms. PRINCIPLES The differentiation of microorganisms on the basis of urea hydrolysis has been widely used for Enterobacteriaceae.1 -2 Urease hydrolizes urea into two ammonia molecules. This causes an increase in the pH which can be detected by the indicator, phenol red. A positive test produces a color change from yellow to pink or red. MATERIALS PROVIDED 10 Test Cupules: Urea 1 Bottle Reagent: Phenol Red and cofactor for Urease Enzyme MATERIALS REQUIRED BUT NOT PROVIDED The standard clinical microbiological equipment such as loop, burner, and incubator are needed for procedures involving the use of this product. PRECAUTIONS This test is intended for use by those trained in appropriate laboratory and bacteriological procedures. This test is for IN VITRO DIAGNOSTIC USE only. Precautions should be taken against the dangers of microbiological hazards. Specimens, containers, media, and test cupules should be sterilized after use. Reagents should not come into contact with skin, eyes, or clothing. Do not inhale or ingest reagents. In case of accident, seek medical attention immediately. STORAGE The ID - Urease kit must be stored at 2-8C to prevent breakdown of the substrates and reagents. EVIDENCE OF DETERIORATION Substrates and reagents provided with this kit should not be used if the expiration date has passed. Do not use this product if a yellow color is not observed upon the addition of the reagent to the substrate cupule. If any deficiencies are noted with this product notify the manufacturer. SPECIMEN COLLECTION Information on specimen collection can be found in reference texts. PROCEDURE 1. Remove cap from cupule and add 10 drops of reagent. Dried substrate may be dissolved by mixing with the inoculating loop or stick in step #2. 2. Inoculate test medium in cup with specimen by selecting 2-3 isolated colonies and mixing with loop to insure uniform suspension of cells. Be sure to mix liquid well at this point with inoculating loop or device to achieve dissolution of substrate. 3. Replace cap and place cupule into reclosable plastic bag or into a rack. 4. Record patient information on bag or on cup if rack is used. 5. Hold at room temperature and read for color changes within 30 minutes.*See Limitation Section INTERPRETATION Positive: Pink to red; urease present Negative: Yellow (No change); urease not present USER QUALITY CONTROL Daily quality control should be performed in accordance with proper laboratory procedures using organisms that will produce known positive and negative reactions. The following American Type Culture Collection strains are recommended: Organism Test Cupule Yersinia enterocolitica (4+) red    ATCC 9610* Proteus mirabilis (2+) red            ATCC 12453* Escherichia coil (-)yellow            ATCC 25922* ATCC is registered trademark American Type Clinical Cultures   LIMITATIONS OF PROCEDURE The substrate medium is very slightly buffered and the yellow color of the medium should not change upon the addition of inoculum. Any color change to pink upon addition which persists over 30 minutes or which develops at a later time may be attributed to urease activity. An inoculum of culture colonies may result in immediate increase in pH and development of a red color due to carryover of extraneous alkaline materials. This red color will fade to yellow and will remain yellow unless urease activity is present. When ID - Urease is used for detection of urease activity in microbial cultures, it should be noted that some microorganisms are more prolific producers of urease than others. Hence, specimens which are negative after 30 minutes should be reexamined at convenient intervals for up to 24 hours. If desired, the cup may be sent to the laboratory for monitoring and/or subculturing. The bag included in the kit provides a convenient transport method. Bacterial overgrowth from other organisms may be present that produce urease causing false positive results. REFERENCES 1. Christensen, W.R., "Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures From Each Other and From Salmonella and Shigella Types," J. Bact. 52:461-466, 1946. 2. Rustigan, R. and C.A. Stuart, "Decomposition of Urea by Proteus," Proc. Soc. Exp. Biol. Med., 47:108-122, 1941. Catalog # 60045

 

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